Posted by
Mohamed Tleis on
May 25, 2014; 5:00pm
URL: http://imagej.273.s1.nabble.com/How-can-we-Distinguish-In-Out-of-focus-cells-in-confocal-microscope-tp5007911p5007914.html
Dear Joel,
Thank you for your reply, My Cells are simple yeast cells. I do Image
Analysis for some images acquired by Biologists. they try to change the
focal level to the most optimal one then they acquire their images.
However, In the analysis part we need to be aware of out of focus cells,
because they can wrongly affect the final measurement results. So my
question is: is there any means to deal with such issues?
Best Regards,
M. Tleis
Phd. Candidate
LIACS, Leiden University
The Netherlands.
On 05/25/2014 06:36 PM, JOEL B. SHEFFIELD wrote:
> Hi Mohamed,
>
> You haven't specified the tissue, but I'll assume that both cells are
> within the z range of the instrument. If it were simply a focus issue, you
> should be able to change the focal level (i.e. do a z scan) and see if you
> get any changes.
>
> Joel
>
>
>
> Joel B. Sheffield, Ph.D
> Department of Biology
> Temple University
> Philadelphia, PA 19122
> Voice: 215 204 8839
> e-mail:
[hidden email]
> URL:
http://astro.temple.edu/~jbs>
>
> On Sun, May 25, 2014 at 9:54 AM, Mohamed Tleis <
[hidden email]> wrote:
>
>> Dear ImageJ members,
>>
>> We have two channel images one of bright-field and one of Green
>> Fluoresence Protein, acquired by a confocal microscope. Recently we are
>> concerned about some cells having less fluoresent due to the fact that it
>> is out of focus. In the bright-field channel it is a bit hard to
>> distinguish between the in and out of focus cells. I wonder whether any of
>> you came across a similar problem or knows of some literature about it.
>>
>> Thank you in Advance for your answers.
>>
>>
>> Best Regards,
>> M. Tleis
>> Phd. Candidate
>> LIACS, Leiden University
>> The Netherlands.
>>
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http://imagej.nih.gov/ij/list.html>>
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