Re: Problem with automated cell counting for DAB stained Fos+ cells in brain slides

Posted by Praz Nina on May 26, 2014; 1:54pm
URL: http://imagej.273.s1.nabble.com/Problem-with-automated-cell-counting-for-DAB-stained-Fos-cells-in-brain-slides-tp5007921p5007926.html

ERIC wrote

Hi Nina,
As you have a large variation of intensity of your nuclei and a quite
homogenous size, it mayhelp to do a frequency filtering.
I transform (eventualy invert) your image in 8bit did an FFT band pass
filter set to 10pixel for larger size and to 4pixel for smaller size
(see FFT filter.png).

//snip

Merci Eric!

This is quite a nice result!

Do you think that this count is pretty similar to the one you would determine by using your eyes?

My manual counting says 239, which means there is around a ~13% error rate, but the question is where - in the automated counting or my subjective counting.

I'm trying to minimize the error rate, so I can analyze a lot of areas of interest in a small amount of time. 13% mistakes would be cumulative, so I'm trying to reduce it as much as I can.