Re: Problem with automated cell counting for DAB stained Fos+ cells in brain slides

Posted by Praz Nina on May 26, 2014; 2:11pm
URL: http://imagej.273.s1.nabble.com/Problem-with-automated-cell-counting-for-DAB-stained-Fos-cells-in-brain-slides-tp5007921p5007927.html

Also, I have tried to use your method on another image (https://i.imgur.com/K9B3FnX.jpg)

But I get something like 5000+ maxima, which doesn't correspond to reality... (my manual counting says 247)

For the automated counting, I don't know if it's possible, but the point would be to use the same settings throughout the section/slide, in order to remove as much variability as possible. Because if I have to change the FFT bandpass for each image independently, that's introducing a new variable..

I've heard things about average threshold measure (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2803710/), but I haven't been able to find the software to test it out (maybe it isn't the best method).