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Re: Problem with automated cell counting for DAB stained Fos+ cells in brain slides

Posted by ERIC on May 26, 2014; 2:38pm
URL: http://imagej.273.s1.nabble.com/Problem-with-automated-cell-counting-for-DAB-stained-Fos-cells-in-brain-slides-tp5007921p5007929.html

Using the same method I found 215 nuclei.
I don't understand where can come your result from. Sorry !!!

I inverted the image after changing it to 8bits.
There is maybe a difference in the binary options ( my settings are
:Black is background)

Eric Denarier
Grenoble Institut des Neurosciences
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France


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Le 26/05/2014 16:11, Praz Nina a écrit :

> Also, I have tried to use your method on another image
> (https://i.imgur.com/K9B3FnX.jpg)
>
> But I get something like 5000+ maxima, which doesn't correspond to
> reality... (my manual counting says 247)
>
> For the automated counting, I don't know if it's possible, but the point
> would be to use the same settings throughout the section/slide, in order to
> remove as much variability as possible. Because if I have to change the FFT
> bandpass for each image independently, that's introducing a new variable..
>
> I've heard things about average threshold measure
> (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2803710/), but I haven't been
> able to find the software to test it out (maybe it isn't the best method).
>
>
>
> --
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> Sent from the ImageJ mailing list archive at Nabble.com.
>
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