Generating random points and tallying proportion of points that fall within object
Posted by atawewe on Jun 02, 2014; 1:54pm
URL: http://imagej.273.s1.nabble.com/Generating-random-points-and-tallying-proportion-of-points-that-fall-within-object-tp5007986.html
I have over 300 images I need to process to obtain an unbiased estimate of the proportion of each image that is occupied by the object of interest. For each image, the objects of interest are black in color, mostly spherical in shape, but range in sizes, while the background of the image is white. The challenge for me is that I need to generate 1000 random points on each image, and determine the proportion of points that fall within the objects of interest in the image. I will appreciate information on how to achieve this using imageJ. After searching online, I really couldn't find any tips on where to start from or how to go about generating random points on an image and estimating whether they fall within the object of interest or not in imageJ.
Thanks in advance.