Re: calibration bar maximum value incorrect?

Posted by Rasband, Wayne (NIH/NIMH) [E] on Jun 05, 2014; 9:35pm
URL: http://imagej.273.s1.nabble.com/calibration-bar-maximum-value-incorrect-tp5008048p5008049.html

On Jun 5, 2014, at 5:13 PM, Neil Fazel wrote:

> I have a 32-bit text image which I have normalized. (I found the maximum pixel value in the image, then divided all pixels in the image by that value.) Then I used Analyze -> Tools -> Calibration Bar... to overlay a calibration bar. I see that the top range of the calibration bar values is not 1, as expected; it's 0.48. The image is normalized; so the brightest pixel's value should be 1. Am I doing something wrong?

Please provide a minimal macro that reproduces the problem, something like the following, except this example works as expected.

-wayne

   run("M51 Galaxy (177K, 16-bits)");
   run("32-bit");
   run("Enhance Contrast...", "saturated=1 normalize");
   run("Fire");
   run("Calibration Bar...", "location=[Upper Right] fill=Black label=White number=5 decimal=1 font=12 zoom=1 bold overlay");

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