Re: calibration bar maximum value incorrect?

Posted by Neil Fazel on Jun 05, 2014; 10:54pm
URL: http://imagej.273.s1.nabble.com/calibration-bar-maximum-value-incorrect-tp5008048p5008053.html

Hi Wayne,

   Thanks for the tip. I hadn't used Process -> Enhance Contrast... to normalize. But I just tried that, and the calibration bar range is correct if I do it that way.

I had done it by first using Analyze -> Measure to find the max pixel value, then by using Process -> Math -> Divide to divide all pixels in the image by the max pixel value. Then I ran the calibration bar. Shouldn't this method also work? I tried it using M51 Galaxy image, and the top range of the color bar is 0.2 instead of 1.

   run("M51 Galaxy (177K, 16-bits)");
   run("32-bit");
   run("Measure");
   run("Divide...", "value=10106");
   run("Calibration Bar...", "location=[Upper Right] fill=Black label=White number=5 decimal=1 font=12 zoom=1 bold overlay");

Best regards,
Neil

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