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Counting and estimating the cells coverage area

Posted by Dakhil on Jun 18, 2014; 11:57am
URL: http://imagej.273.s1.nabble.com/Counting-and-estimating-the-cells-coverage-area-tp5008276.html

I am suffering from counting and estimating the cells coverage area in my bright field images. I am trying with imagej but I have no clue it is the right way to count the cells and roughly estimate the coverage area of these cells. Firstly, I am doing trial and error auto local threshold with different parameters until get something better then using the Analyze particles but it still unbelievable data. Could you please give me a piece of advise to overcome this big issue. I uploaded a sample of my images.

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