Re: Counting and estimating the cells coverage area

Posted by Paul van Schayck on Jun 19, 2014; 5:50am
URL: http://imagej.273.s1.nabble.com/Counting-and-estimating-the-cells-coverage-area-tp5008276p5008306.html

Dear Dakhil,

Even from the image I have trouble seeing what are cells and what are
not. Is it possible to improve the contrast during acquisition?

Kind regards,

Paul

On Wed, Jun 18, 2014 at 1:57 PM, Dakhil
<[hidden email]> wrote:

> I am suffering from counting and estimating the cells coverage area in my
> bright field images. I am trying with imagej but I have no clue it is the
> right way to count the cells and roughly estimate the coverage area of these
> cells. Firstly, I am doing trial and error auto local threshold with
> different parameters until get something better then using the Analyze
> particles but it still unbelievable data. Could you please give me a piece
> of advise to overcome this big issue. I uploaded a sample of my images.
>
> 12.jpg <http://imagej.1557.x6.nabble.com/file/n5008276/12.jpg>
>
>
>
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