Re: Counting and estimating the cells coverage area
Posted by Dakhil on Jun 19, 2014; 9:17am
URL: http://imagej.273.s1.nabble.com/Counting-and-estimating-the-cells-coverage-area-tp5008276p5008309.html
Dear Paul,
Thanks for your interest. Unfortunately, the objective and the camera has been placed underneath a glass plate with thickness of 11 mm and the bright light source through the objective tube. The cells are 3T6 fibroblasts and I am measuring the rheological properties under oscillatory rheometer in parallel-disk configuration. The cells density are so high, more than 90% confluence and I have to calculate the cell coverage area and number of cells per each image have been taken.
Regards.
Haider