Re: Create ROI by automatically wrapping low spatial moment areas

Posted by dscho on Jun 24, 2014; 1:38pm
URL: http://imagej.273.s1.nabble.com/Create-ROI-by-automatically-wrapping-low-spatial-moment-areas-tp5008387p5008394.html

Hi Giuseppe,

On Mon, 23 Jun 2014, giuseppe3 wrote:

> I need to create a new ROI which outlines the area with high ratios and
> save it so that for each cell I have an ROI for the whole cell and for
> the "inner cytosol".

What you typically do in such a case is to duplicate the original, set a
threshold and create a selection, then close the duplicate and restore the
selection.

If you need the new selection only inside another ROI, you have to do the
following steps instead of creating the selection from the threshold:
create a binary from the threshold, then duplicate the original once more,
apply the ROI, create a mask, and then perform the AND operation on both
masks via the Image Calculator.

Good luck,
Johannes

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html