Re: Create ROI by automatically wrapping low spatial moment areas

Posted by Giuseppe Lucarelli on Jun 24, 2014; 11:47pm
URL: http://imagej.273.s1.nabble.com/Create-ROI-by-automatically-wrapping-low-spatial-moment-areas-tp5008387p5008414.html

Thanks Johannes, I will try your suggestion.

Also I thought of using the wand tool to select the area by finding the maxima first, then set the tolerance for the wand depending on how the value of the maxima.

This may work better but I need to find out how to count the number of maxima per ROI (cell) and go through each of them and apply the wand tool.

What do you think?

Also I am not 100% sure on what steps I need to make after reading your comment:

> If you need the new selection only inside another ROI, you have to do the
> following steps instead of creating the selection from the threshold:
> create a binary from the threshold, then duplicate the original once more,
> apply the ROI, create a mask, and then perform the AND operation on both
> masks via the Image Calculator.

Thanks,

Giuseppe