Posted by
Gabriel Landini on
Jun 27, 2014; 12:39pm
URL: http://imagej.273.s1.nabble.com/Contrast-brightness-in-selected-areas-tp5008461p5008468.html
> > -----Original Message-----
> > I have a TEM picture which is composed from 4 collectors and merged
> > automatically into one picture. However, the setup is not calibrated
> > properly, leading to different contrast/brightness for the different
> > collectors. Therefore I am trying to go through the four sub pictures one
> > by one and adjust the contrast so that I get a nice final picture
Further to the good previous advice, the problem is that your background is
not even, so just adjusting brightness to 2 adjacent frames probably does not
make the other adjacent frames match.
One thing you can do is to convert your montage to a stack as suggested, then
try to 'flatten' the background of your images using some background
correction method and remake the montage.
You should read this (the "retrospective" section):
http://imagejdocu.tudor.lu/doku.php?id=howto:working:how_to_correct_background_illumination_in_brightfield_microscopythen try some of the "a posteriori" methods, but always having in mind that
will be degrading the image contents by making assumptions on how the
background behaves, when we do not know how correct that is. The images might
look better, but the image data might become artificially biased (eg, if you
are intending to measure intensities).
Good luck,
Gabriel
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