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Re: Tif Stacks Not Opening Correctly

Posted by ctrueden on Jul 14, 2014; 8:18pm
URL: http://imagej.273.s1.nabble.com/Tif-Stacks-Not-Opening-Correctly-tp5008677p5008728.html

Hi Dan,

> I just uploaded it.  Sorry for the delay.

No worries, we received the file and can reproduce the problem. Both SCIFIO
and Bio-Formats (which have very similar TIFF reading code) only identify
20 planes instead of the full 23. I filed an issue:

https://github.com/scifio/scifio/issues/212

We will investigate and fix when we can.

Regards,
Curtis


On Mon, Jul 14, 2014 at 12:00 PM, J. Daniel Fenn <
[hidden email]> wrote:

>  Hi Curtis,
>
> I just uploaded it.  Sorry for the delay.
>
> I didn't get any sort of confirmation that it uploaded.  The file was
> named 02-4_PE.tif
>
> Let me know if I can help out in any other way.
>
> Best,
> -Dan
>
> If it didn't upload I can maybe try sending it to you directly.  I don't
> know how to tell for sure if it uploaded or not.
>
>
> On 07/14/2014 12:23 PM, Curtis Rueden wrote:
>
> Hi Dan,
>
>  > I will upload a sample stack later today.
>
>  Did you get a chance to upload a sample TIFF yet? I don't see any new
> recent uploads since your mail.
>
>  > But when I open the stack in Fiji, it only finds 20 of the 23 images.
>
>  I would really like to investigate this bug, since we have had at least
> one other report of "missing image planes" similar to this.
>
>  Thanks,
> Curtis
>
>
> On Fri, Jul 11, 2014 at 7:15 AM, J. Daniel Fenn <
> [hidden email]> wrote:
>
>>  Curtis,
>>
>> It does appear to open correctly when I turn off SCIFIO.  I will upload a
>> sample stack later today.  Thanks!
>>
>> -Dan
>>
>>
>> On 07/10/2014 11:41 PM, Curtis Rueden wrote:
>>
>> Hi J. Daniel,
>>
>>  > I have discovered that the images open fine in older version of Fiji
>> > (the life-line versions).  The older versions I get all 23 planes as
>> > expected.
>>
>>  Please try running Edit > Options > ImageJ2 and disabling the "Use
>> SCIFIO when opening files" option, then opening your TIFFs again. Do they
>> open correctly? If so, it is a problem with the new SCIFIO support.
>>
>>  If that is the case, it would be very helpful if you could send a
>> sample non-working TIFF file using the Help > Upload Sample Image command.
>>
>>  Thanks,
>> Curtis
>>
>>
>> On Thu, Jul 10, 2014 at 3:28 PM, J. Daniel Fenn <
>> [hidden email]> wrote:
>>
>>> I'm curious if anyone has run in to this problem.
>>>
>>> I have a stack saved as a .tif image (multi-page tif).  The stack has 23
>>> images/pages within the stack.  The stack (all 23 images) open fine on
>>> any imaging program I have access to (Gimp, photoshop, exiv2, etc).
>>>
>>> But when I open the stack in Fiji, it only finds 20 of the 23 images.  I
>>> have this same problem on multiple images, but each one is slightly
>>> different.  Some are missing 3 planes, some are missing 1, some are
>>> missing 10 planes.  Again, on all other imaging software all planes show
>>> up.
>>>
>>> I have no idea where to even begin to troubleshoot this problem.  Does
>>> anyone have any ideas?  I have discovered that the images open fine in
>>> older version of Fiji (the life-line versions).  The older versions I
>>> get all 23 planes as expected.
>>>
>>> Am I doing something wrong?
>>>
>>> --
>>> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>>>
>>
>>
>>
>
>

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