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Re: Measuring mulitple species from a single image

Posted by vischer on Jul 15, 2014; 5:55am
URL: http://imagej.273.s1.nabble.com/Measuring-mulitple-species-from-a-single-image-tp5008690p5008736.html

Hello Gail,

> On 11. Jul 2014, at 21:51, gaton <[hidden email]> wrote:
>
> Hello,
> I'm trying to measure (area), categorise (as species) and count multiple
> individuals from a single image. E.g. image of a fouling community with
> multiple individuals of different species- I want to know how many species,
> how many individuals of each species, and area covered by each species or
> individual.
> I'm using the Wand tool to select the individuals as ROIs- adding these to
> the ROI manager. I could then rename them as species, measure, export the
> results to excel and count from there. But I'm wondering if there is some
> way to automate the naming- e.g. have 10 species each assigned to a number
> key and when I press that key it renames the most recent ROI (I'll panic
> when I go over 10 species, but you get the gist). Or- using different colors
> to highlight the different species and then somehow recording the selection
> color as a column in the measurement results (again, export to excel & count
> colors from there).
> I've also added ObjectJ which does most of the above, except I can't use the
> wand tool from within ObjectJ.
>
>
> Does anyone have any suggestions of a work-around?
>
> Thanks,
> Gail

You can look at this ObjectJ example which uses shortcut keys to create outlines of different categories and sorts the results into different columns.
http://simon.bio.uva.nl/objectj/examples/RoiCounter/MD/roicounter.html

Norbert Vischer
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