Re: Measuring mulitple species from a single image

Posted by vischer on Jul 16, 2014; 9:28pm
URL: http://imagej.273.s1.nabble.com/Measuring-mulitple-species-from-a-single-image-tp5008690p5008784.html

Hi Gail,

the idea was that you need not to use the wand at all. Instead, locate the cursor without clicking the mouse, then press the key [1], [2] or [3]. It will  create the roi (just as the wand would do), and directly converts it into an object of the desired type.

Make sure not to mark the same roi twice, as two equivalent rois above each-other are only distinguishable by the superimposition of two different number labels.

regards, Norbert
On 15. Jul 2014, at 19:57, gaton <[hidden email]> wrote:

> Hi Norbert,
> The description of ROIcounter on the link you sent sounded perfect. Either I
> am not using it correctly, or I misinterpreted the description. I thought I
> would be able to hover over ROIs I had already created using the Wand, press
> [1] and have them stored as Object Type1. But instead, I have to select
> Type1, then still draw around the ROI using the ObjectJ Marker Tool in order
> to define the area.
> The problem with the ROIcounter/Cellcounter in ObjectJ is that you have to
> manually draw around the ROI- not select it using the wand tool- which is
> much more accurate for outlining an individual (given my mouse manipulation
> skills).
> Any other suggestions?
> Thanks,
> Gail
>

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