Re: Measuring Fluorescence density of nuclei

Posted by nmichae on Jul 23, 2014; 6:41pm
URL: http://imagej.273.s1.nabble.com/Measuring-Fluorescence-density-of-nuclei-tp5008116p5008868.html

Hi,

I was also using the same function for measuring the density of activated area in the brain. What I did is, I kept the area chosen constant between the different images, using the command (ctrl+shift+E). This transferred the same ROI to a different image so that I could measure the density of the same area in different images using ctrl+M command afterwards.
But now I have an issue. What is actually the numner which we are getting when we give measure command? if the number which we get is directly proportional to the density of the ROI, it is not matching. Beacuse where I expect higher density (clear even with mere eye inspection), I get a smaller number where as where there lower density I get a high value. So what am I actually calculating? Im chosing -- set measurements--mean grey value.

regards,

Neethu