Retina vessel segmentation

Posted by Mr_Sonky on Jul 24, 2014; 4:03am
URL: http://imagej.273.s1.nabble.com/Retina-vessel-segmentation-tp5008869.html

Hi, I am new to ImageJ/Fiji and am also new to this forum. I'm currently working on a way to segment some blood vessels from a 16-bit grayscale image of a rodent retina. See image below:



I have tried all auto-threshold methods and manual thresholding but still no dice. The areas adjacent to the vessel have pixel values too similar to my vessel. Yet I can easily demarcate these vessels with my naked eye (manual segmentation)..

I've done a quick search of the forum but did not find anything useful... any help is appreciated!