Re: Measuring Fluorescence density of nuclei

Posted by nmichae on
URL: http://imagej.273.s1.nabble.com/Measuring-Fluorescence-density-of-nuclei-tp5008116p5008877.html

Hello Oliver,

Thank you for the message. But what Im doing is not fluroescence measurement but we do optical imaging. and we get the neuronal activity in a grey scale activity map. So my intention was to measure the density of this activity patch. So where i expect a high density or where I have a darker activity patch, which denotes high brain activity, I 'm getting a small measurement value when I use this tool in imageJ. Thats why I was wondering, what actually is being measured here. Im very bad in explaining. Dont know whether you will understand what Im trying to say.

Regards,

Neethu