Re: Retina vessel segmentation

Posted by James Ewing on Jul 24, 2014; 12:45pm
URL: http://imagej.273.s1.nabble.com/Retina-vessel-segmentation-tp5008869p5008881.html

Your eye is looking at the edges and other relatively high-frequency (spatial) phenomena.  You might try a Fourier transform, filter the low-frequency components, and back-transform.  Alternatively, there are spatial filters that are basically high-pass filters - try a Gaussian high-pass filter.
  - Jim Ewing

On Jul 24, 2014, at 12:03 AM, Mr_Sonky <[hidden email]> wrote:

> Hi, I am new to ImageJ/Fiji and am also new to this forum. I'm currently
> working on a way to segment some blood vessels from a 16-bit grayscale image
> of a rodent retina. See image below:
>
> <http://imagej.1557.x6.nabble.com/file/n5008869/Eye_blood_vessel.png>
>
> I have tried all auto-threshold methods and manual thresholding but still no
> dice. The areas adjacent to the vessel have pixel values too similar to my
> vessel. Yet I can easily demarcate these vessels with my naked eye (manual
> segmentation)..
>
> I've done a quick search of the forum but did not find anything useful...
> any help is appreciated!
>
>
>
>
>
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