3DObject Counter Surface Map

Posted by Nadia Cummins on Jul 25, 2014; 3:03am
URL: http://imagej.273.s1.nabble.com/3DObject-Counter-Surface-Map-tp5008891.html

Dear ImageJ Users,
Im trying to use the 3d object counter to analyse mitochondria. While the plug in seems to run through its steps just fine, the generated surface map at the end looks nothing like what I would expect looking at the thresholded image. Instead of displaying the rendered surfaces (which I need so I can compare the analysis/segmentation with my original image) it just has tiny dots, similar to what I would expect for the "centroids" to look like.

Have I misunderstood the descriptions of the various maps generated? How can I be confident that the analysis has picked up the mitochondria correctly if I cant compare the final segmented "image" with the original?

Thanks, Nadia

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