Setting custom measurement for scale bar in use for measuring fungal spores and other microscopic details of fungi

Posted by Andy Overall on Jul 25, 2014; 3:17pm
URL: http://imagej.273.s1.nabble.com/Setting-custom-measurement-for-scale-bar-in-use-for-measuring-fungal-spores-and-other-microscopic-dei-tp5008899.html

>>> I am new to ImageJ and have been trying utilise the programme to set up my own scale bar set to 10 microns, to be able to measure fungal spore with it. I have already photographed the scale bar at a x1000 on my microscope and have opened it in ImageJ.  I was able to create a 10micron scale bar for comparing an spores against but I need the exact measurements to actually appear when I measure the spores.  Would it be possible for somebody to please clearly explain the process that I need to follow, to be able to achieve this?
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