Re: Setting custom measurement for scale bar in use for measuring fungal spores and other microscopic details of fungi

Posted by Andy Overall on Jul 25, 2014; 5:53pm
URL: http://imagej.273.s1.nabble.com/Setting-custom-measurement-for-scale-bar-in-use-for-measuring-fungal-spores-and-other-microscopic-dei-tp5008899p5008908.html

Thanks Kees,

Yes, when I was trying measure the spore, that is exactly what I seeing, just angle and length. When you say scaled image......I assume that is following having managed to set up my scale, measurement, which is what I was having a problem doing.  Once I have managed to this, then I shall try what you say.

Many Thanks
Andy
 
On 25 Jul 2014, at 17:53, Straatman, Kees (Dr.) wrote:

> Dear Andy,
>
> Once you have a scaled image you can use the straight line Tool to draw a line from one side of the spore to the other. Press 'm' and you should see a result appearing. Repeat to do more measurements. You can deselect everything under Analyze > Set Measurements and the results will only show you Angle and Length of your line.
>
> Good luck
>
> Kees
>
>
>
> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Michael Schmid
> Sent: 25 July 2014 17:39
> To: [hidden email]
> Subject: Re: Setting custom measurement for scale bar in use for measuring fungal spores and other microscopic details of fungi
>
> Hi Andy,
>
> there are several web pages explaining how to set the image scale; some of these even have a link in the ImageJ documentation:
> http://rsbweb.nih.gov/ij/docs/guide/146-30.html#toc-Subsection-30.8
>
> Others are e.g.
> http://microscopy.berkeley.edu/courses/dib/sections/04IPIII/IJsetscale.html
> http://serc.carleton.edu/eyesinthesky2/week2/get_to_know_imagej.html#find_scale
> http://skepticwonder.fieldofscience.com/2009/11/quick-imagej-tutorial-scalebar.html
> http://wiki.imagej.net/SpatialCalibration
>
> And any internet search engine will give you dozens of others...
>
> Michael
> ________________________________________________________________
>
>
> On Jul 25, 2014, at 17:17, Andy Overall wrote:
>
>> I am new to ImageJ and have been trying utilise the programme to set up my own scale bar set to 10 microns, to be able to measure fungal spore with it. I have already photographed the scale bar at a x1000 on my microscope and have opened it in ImageJ.  I was able to create a 10micron scale bar for comparing an spores against but I need the exact measurements to actually appear when I measure the spores.  Would it be possible for somebody to please clearly explain the process that I need to follow, to be able to achieve this?
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html