http://imagej.273.s1.nabble.com/Setting-custom-measurement-for-scale-bar-in-use-for-measuring-fungal-spores-and-other-microscopic-dei-tp5008899p5008923.html
Very helpful. The Q value is the ratio of length/breadth for a single spores and the observed range of the ratio of length/breadth for all spores measured.
> Dear Andy,
>
> If you right mouse-click in the results table you get several option including "Summarize" what will add Mean, SD, Min and Max to your results table, no Q-value. Is this Q-value related to FDR or something else?
>
> Best wishes
>
> Kees
> -----Original Message-----
> From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Andy Overall
> Sent: 26 July 2014 09:34
> To:
[hidden email]
> Subject: Re: Setting custom measurement for scale bar in use for measuring fungal spores and other microscopic details of fungi
>
> Thanks Kees,
>
> I'm up and running I love the way that all of the measurements are kept in the pop up box, would I also be able to ascertain average sizes for say 20 spores and obtain a Q value?
>
> Thanks
>
> Andy
>
> On 25 Jul 2014, at 17:53, Straatman, Kees (Dr.) wrote:
>
>> Dear Andy,
>>
>> Once you have a scaled image you can use the straight line Tool to draw a line from one side of the spore to the other. Press 'm' and you should see a result appearing. Repeat to do more measurements. You can deselect everything under Analyze > Set Measurements and the results will only show you Angle and Length of your line.
>>
>> Good luck
>>
>> Kees
>>
>>
>>
>> -----Original Message-----
>> From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of
>> Michael Schmid
>> Sent: 25 July 2014 17:39
>> To:
[hidden email]
>> Subject: Re: Setting custom measurement for scale bar in use for
>> measuring fungal spores and other microscopic details of fungi
>>
>> Hi Andy,
>>
>> there are several web pages explaining how to set the image scale; some of these even have a link in the ImageJ documentation:
>>
http://rsbweb.nih.gov/ij/docs/guide/146-30.html#toc-Subsection-30.8>>
>> Others are e.g.
>>
http://microscopy.berkeley.edu/courses/dib/sections/04IPIII/IJsetscale>> .html
>>
http://serc.carleton.edu/eyesinthesky2/week2/get_to_know_imagej.html#f>> ind_scale
>>
http://skepticwonder.fieldofscience.com/2009/11/quick-imagej-tutorial->> scalebar.html
http://wiki.imagej.net/SpatialCalibration>>
>> And any internet search engine will give you dozens of others...
>>
>> Michael
>> ________________________________________________________________
>>
>>
>> On Jul 25, 2014, at 17:17, Andy Overall wrote:
>>
>>> I am new to ImageJ and have been trying utilise the programme to set up my own scale bar set to 10 microns, to be able to measure fungal spore with it. I have already photographed the scale bar at a x1000 on my microscope and have opened it in ImageJ. I was able to create a 10micron scale bar for comparing an spores against but I need the exact measurements to actually appear when I measure the spores. Would it be possible for somebody to please clearly explain the process that I need to follow, to be able to achieve this?
>>
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