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automated counting of triple labeled cells

Posted by Liz Bless on Aug 06, 2014; 8:21pm
URL: http://imagej.273.s1.nabble.com/automated-counting-of-triple-labeled-cells-tp5009050.html

Hi -

I have IHC images that contain 3 labels. I would like imageJ to be able to automatically count 1) the number of red cells in the image, 2) the number of red cells that are also blue, 3) the number of red cells that are also blue and green.

I know how to count single labeled cells but could anyone direct me to a plugin for counting double and triple labelled cells or advise me on how to go about this?

Thank you!
Liz

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