Posted by
LIM Soon Yew John (IMB) on
Aug 07, 2014; 2:16am
URL: http://imagej.273.s1.nabble.com/FIJI-thinks-my-greyscale-image-stack-is-a-coloured-image-tp5009051p5009053.html
Hi Sonky,
Are you using FIJI (ImageJ2)? If yes, you can try to turn off the SCIFIO under Edit>Options>ImageJ2. I encounter an issue that FIJI (ImageJ2) did not open my RGB image correctly but it works again after I turn off SCIFO.
Another thing that you can try is go to Image>Hyperstacks>Stack to Hyperstack..., set the correct number of slices and channel.
Best Regards,
John
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________________________________________
From: ImageJ Interest Group [
[hidden email]] On Behalf Of Mr_Sonky [
[hidden email]]
Sent: Thursday, August 07, 2014 9:56 AM
To:
[hidden email]
Subject: FIJI thinks my greyscale image stack is a coloured image?
Hi all, I've got a simple question. Why does FIJI open my stack of grayscale
images as a colour image?? I know it does this because of the letter "c" in
place of the "play" button when we want to scroll between slices in a stack.
Also it does this annoying thing where it colour codes i.e. Slice #1: Red,
Slice #2: Green..etc.. this goes on to Slice #6: Yellow.. and Slice 7
onwards to Slice 361 remains gray (its original colour). All images are
acquired by a monochromatic sensor so I know the colour is not real.
When I apply a 'Grays' LUT, it either doesn't save or I wants me to convert
to 8-bits first. But doing so doesnt solve the problem of the "c" slider.
In addition, I found that when the "c" slider is present, I can't adjust the
Brightness/Contrast for all slices whereas before I normally could with a
standard greyscale stack. *When I tried opening the SAME image file using
ImageJ, it opens normally* without the pseudo-colouring the first 6 slices.
Everyhting else also works when opened in ImageJ (as opposed to FIJI)
<
http://imagej.1557.x6.nabble.com/file/n5009051/Sample_image.png>
The image on the right and left essentially the same opened in different
programs.. Would really appreciate if someine can explain to me how to stop
this annoying behaviour!
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