Re: Coloc2 - quantifying colocalization

Posted by Sylwia J on
URL: http://imagej.273.s1.nabble.com/Coloc2-quantifying-colocalization-tp5008964p5009091.html

Hi Chong,

That's really helpful, thank you! Would you be so kind to briefly explain to me a bit more about PSF? As I haven't used ImageJ before and thus don't know what exactly it is. I tried to follow your suggestion (Colocalisation > Colocalisation Test) to estimate PSF but am not sure how. I chosen Randomisation method > Costes approximation (smoothed noice) and then a window appeared: PSF details (as below)



When I choose Analyze > Colocalisation > Coloc2 it automatically assignes PSF 10 and Costes 10 (as below), so that's why I assumed this was correct



Also, do I really need Costes if I want to look at Pearson's or Mander's coefficients?


Thanks so much for your help!

Sylwia