Re: Analyze DF/F

Posted by Krs5 on Aug 13, 2014; 8:01am
URL: http://imagej.273.s1.nabble.com/Analyze-DF-F-tp5009145p5009160.html

Dear Nicolas,

To correct for small movement artefacts (jittery) you could use the Image Stabilizer plugin (http://www.kangli.org/code/Image_Stabilizer.html) or TurboReg plugin (http://bigwww.epfl.ch/thevenaz/turboreg/)

I think without a few sample frames it will be difficult for list members to help with the ROI selection.

Best wishes

Kees

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Nicolás Pírez
Sent: 12 August 2014 18:49
To: [hidden email]
Subject: Analyze DF/F

Dear users,

I would like to be able to measure the intensity change in a cell body along a time lapse.
I would like to first threshold the image and from that threshold image run a plugging to measure the intensity change (multimeasure ??).

I am having trouble being able to automatically select the ROI from the threshold image. Additionally I would like to be able to correct for small movement artifacts.

Any help would be greatly appreciated. Let me know if an example image would be necessary but a bit big.
​
Thanks, Nicolas.​

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Nicolás Pírez, PhD
[hidden email]
[phone]

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