Posted by
Nicolás Pírez on
Aug 13, 2014; 12:26pm
URL: http://imagej.273.s1.nabble.com/Analyze-DF-F-tp5009145p5009170.html
Dear Kees, thanks a lot for your response. I will look at those plugins!
Here I am attaching four consecutive frames that I hope serve as an example
for the ROI I am working on.
Thanks a lot.
Nico.
--
Nicolás Pírez, PhD
[hidden email]
+5491164161970
On Wed, Aug 13, 2014 at 5:03 AM, Straatman, Kees (Dr.) <
[hidden email]
> wrote:
> Dear Nicolas,
>
> To correct for small movement artefacts (jittery) you could use the Image
> Stabilizer plugin (
http://www.kangli.org/code/Image_Stabilizer.html) or
> TurboReg plugin (
http://bigwww.epfl.ch/thevenaz/turboreg/)
>
> I think without a few sample frames it will be difficult for list members
> to help with the ROI selection.
>
> Best wishes
>
> Kees
>
> -----Original Message-----
> From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of
> Nicolás Pírez
> Sent: 12 August 2014 18:49
> To:
[hidden email]
> Subject: Analyze DF/F
>
> Dear users,
>
> I would like to be able to measure the intensity change in a cell body
> along a time lapse.
> I would like to first threshold the image and from that threshold image
> run a plugging to measure the intensity change (multimeasure ??).
>
> I am having trouble being able to automatically select the ROI from the
> threshold image. Additionally I would like to be able to correct for small
> movement artifacts.
>
> Any help would be greatly appreciated. Let me know if an example image
> would be necessary but a bit big.
>
> Thanks, Nicolas.
>
> --
> Nicolás Pírez, PhD
>
[hidden email]
> +5491164161970
>
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