> Dear Kees, thanks a lot for your response. I will look at those plugins!
>
> Here I am attaching four consecutive frames that I hope serve as an example
> for the ROI I am working on.
>
> Thanks a lot.
>
> Nico.
>
>
> --
> Nicolás Pírez, PhD
>
[hidden email]
> +5491164161970
>
>
> On Wed, Aug 13, 2014 at 5:03 AM, Straatman, Kees (Dr.) <
>
[hidden email]
> > wrote:
>
> > Dear Nicolas,
> >
> > To correct for small movement artefacts (jittery) you could use the Image
> > Stabilizer plugin (
http://www.kangli.org/code/Image_Stabilizer.html) or
> > TurboReg plugin (
http://bigwww.epfl.ch/thevenaz/turboreg/)
> >
> > I think without a few sample frames it will be difficult for list members
> > to help with the ROI selection.
> >
> > Best wishes
> >
> > Kees
> >
> > -----Original Message-----
> > From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of
> > Nicolás Pírez
> > Sent: 12 August 2014 18:49
> > To:
[hidden email]
> > Subject: Analyze DF/F
> >
> > Dear users,
> >
> > I would like to be able to measure the intensity change in a cell body
> > along a time lapse.
> > I would like to first threshold the image and from that threshold image
> > run a plugging to measure the intensity change (multimeasure ??).
> >
> > I am having trouble being able to automatically select the ROI from the
> > threshold image. Additionally I would like to be able to correct for
> small
> > movement artifacts.
> >
> > Any help would be greatly appreciated. Let me know if an example image
> > would be necessary but a bit big.
> >
> > Thanks, Nicolas.
> >
> > --
> > Nicolás Pírez, PhD
> >
[hidden email]
> > +5491164161970
> >
> > --
> > ImageJ mailing list:
http://imagej.nih.gov/ij/list.html> >
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> > ImageJ mailing list:
http://imagej.nih.gov/ij/list.html> >
>
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