Re: Pixels to squared microns

Posted by marcosfelipeti on Aug 13, 2014; 7:56pm
URL: http://imagej.273.s1.nabble.com/Pixels-to-squared-microns-tp5009142p5009187.html

Hi Pariksheet,

Thank you very much for answering! I found out that the pictures I have are in PNG format, so I converted them to TIFF format and now I can see some information. See an image sample below:

Dimensions: 498 x 109
Horizontal and Vertical resolution: 90dpi
Bit depth: 32

Online found: To convert dpi to microns: 25,400/dpi = microns, that is, 25,400/90dpi = 282.2 um

I also found : 90 dot/inch  =  0.0035433071 pixel/micrometer

I just don't know the values that I should use to calibrate the image for all the images, since all of them have 90dpi.

Thank you.