watershed

Posted by Pamela Young on Aug 15, 2014; 3:07am
URL: http://imagej.273.s1.nabble.com/watershed-tp5009231.html

I was giving a FIJI demo yesterday and noticed that

Process>Binary>Watershed

separated joined particles. But if I inverted the LUT, it connects the particles.

I was playing with the Blobs file and just threw a quick threshold on to make a mask.  So the blobs were 255 and the background was 0.  But I didn’t like that the blobs were visually black and the background was visually white.  So I clicked Invert LUT.  The blobs were still 255 and the background was still 0.  But with the new LUT watershed connects all the blobs.  Why does the LUT matter?

THANKS!


Dr Pamela A. Young | Light and Optical Microscopist
Australian Centre for Microscopy & Microanalysis

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