> In trying to segment/track my cells using the ABSnake plugin, I get the
> error posted below. OS is 64bit Win7, 32GB memory, and my image stack is a
> 16-bit .lsm confocal timelapse in one color/channel only (2D+t), opened by
> the non-SCIFIO option. I get the same results if I convert to 8-bit first.
> Seems like it can't open the second image for some reason.
>
> Options used in plugin were all the defaults, except I ticked "create seg
> image." Tried it without that option also, similar results. Also tried with
> a single ROI instead of 16, to no avail. ROIs were created by thresholding
> then magic wand.
>
> Also, image is reported as "locked' after this error, which I've never
> encountered. I have to restart the whole process to tinker with it.
>
> Tried to give all necessary info, please forgive if I've forgotten
> something.
>
> JPK
>
>
> Log:
>
> processing slice 1 with roi 0
> processing slice 1 with roi 1
> processing slice 1 with roi 2
> processing slice 1 with roi 3
> processing slice 1 with roi 4
> processing slice 1 with roi 5
> processing slice 1 with roi 6
> processing slice 1 with roi 7
> processing slice 1 with roi 8
> processing slice 1 with roi 9
> processing slice 1 with roi 10
> processing slice 1 with roi 11
> processing slice 1 with roi 12
> processing slice 1 with roi 13
> processing slice 1 with roi 14
> processing slice 1 with roi 15
>
>
> Error Message:
>
> (Fiji Is Just) ImageJ 2.0.0-rc-14/1.49f; Java 1.6.0_24 [64-bit]; Windows 7
> 6.1; 1869MB of 24465MB (7%)
>
> java.lang.NegativeArraySizeException
> at ij.gui.PolygonRoi.<init>(PolygonRoi.java:39)
> at ABSnake.createRoi(ABSnake.java:216)
> at ABSnake_.run(ABSnake_.java:211)
> at
> ij.plugin.filter.PlugInFilterRunner.processOneImage(PlugInFilterRunner.java:262)
> at ij.plugin.filter.PlugInFilterRunner.<init>(PlugInFilterRunner.java:111)
> at ij.IJ.runUserPlugIn(IJ.java:204)
> at ij.IJ.runPlugIn(IJ.java:166)
> at ij.Executer.runCommand(Executer.java:131)
> at ij.Executer.run(Executer.java:64)
> at java.lang.Thread.run(Thread.java:662)
>
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