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Re: detection of fatty tissue in HE images

Posted by Volker Baecker on Aug 29, 2014; 8:50am
URL: http://imagej.273.s1.nabble.com/detection-of-fatty-tissue-in-HE-images-tp5009413p5009415.html

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Hello,
I did something similar, see

http://dev.mri.cnrs.fr/projects/imagej-macros/wiki/Adipocytes_Tool

You will need to find the right parameters and maybe adapt the macros
to your needs.

Alternatively there is

Osman OS, Selway JL, K?pczy?ska MA, Stocker CJ, O’Dowd JF, Cawthorne
MA, et al. A novel automated image analysis method for accurate
adipocyte quantification. Adipocyte. 2013 Jul 1;2(3):0–1.

using MATLAB

Best regards,
Volker Baecker

Marien Koen wrote:
> Dear ImageJ mailing list
>
>
> Currently I'm trying to write an algorithm that can detect fatty
> tissue in an image of HE stained tissue (examples:
> https://www.dropbox.com/sh/40unigstsq795tk/AADpFIvIbudvDCJqdNxINa1_a?dl=0).
>
>
Making a difference between holes in the tissue and the fatty is not easy.

>
> Can somebody help me with a succesful workflow? With choosing the
> right filters? Or if you know good literature about this?
>
>
> Thanks Koen
>
>
> ir. Koen Marien
>
> Imaging Scientist, PhD student
>
> <file://hgx-storage2/General/Templates/HGX%20logooke.jpg>
>
> Lab building, 1st floor (campus ZNA Middelheim)
>
> Lindendreef 1, 2020 Antwerpen (Belgium)        Phone: +32 (0)3 218
> 19 17
>
> https://www.uantwerp.be/en/staff/koen-marien/
>
> -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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