.ND2 reader in BioFormats

Posted by lechristophe on Sep 05, 2014; 2:42pm
URL: http://imagej.273.s1.nabble.com/ND2-reader-in-BioFormats-tp5009525.html

Hi,

This is primarily a BioFormat question.

I'm trying to open a big Nikon .nd2 files in Fiji using BioFormats (not the
new SciFiIO) in Fiji, Windows 7 64 bits, 64GB RAM. The "Reading file header
part" usually peaks well above the actual file size with 17.6 GB (on ImageJ
memory monitor) for a 6.3 GB nd2 file. For a 15.9 GB file, it maxes out the
60 GB allocated to Fiji. As it is the header reading part, it does not make
a difference to try to open as a virtual stack or not.

Is this a known situation? What are my options for opening these files?

Thanks for your help,

Christophe

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