Errors when converting from 32-bit uncompressed TIF to 16- or 8-bit?

Posted by Leonardo Guizzetti on Sep 13, 2014; 12:14am
URL: http://imagej.273.s1.nabble.com/Errors-when-converting-from-32-bit-uncompressed-TIF-to-16-or-8-bit-tp5009610.html

Hello all,

I think this message belongs here. I've noticed that when I generated an
uncompressed 32-bit TIF using Python, then open it in FIJI, the image will
eventually be converted (in FIJI) to a 16-bit. However, in a test image
which only has pixels valued at 0 or 100, when I downsample, the dynamic
range is expanded to fill the new bit-depth. My pixel values now max out at
65535 or 256. Is this an error, or if not, what is the proper way to shrink
the bit-depth of my images?

I have uploaded a sample image using FIJI, called "points.tif" which you
can use to verify the problem.

Thanks,

Lenny

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