threshold

Posted by Sarah Mohamed on Sep 16, 2014; 10:15am
URL: http://imagej.273.s1.nabble.com/threshold-tp5009636.html

Hi,
Can I manipulate with the image brightness before analysis using threshold?


I am trying to determine brown area stained with DAB using image J, i
tried to use threshold and color deconvolution. my images a dark can i
use photoshop to adjust background or adjust brightness (using levels or curves) ? or that will change the results?


Regards,Sarah M. Mosaad, M.Sc.

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