Re: 4 channel png files

Posted by Christian Goosmann-2 on Sep 16, 2014; 1:16pm
URL: http://imagej.273.s1.nabble.com/4-channel-png-files-tp5009630p5009642.html

Dear Larry
try to open all images in the folder with 'File>import>image sequence...'.
You should get all images in one stack. Then use
'Image>Hyperstacks>Stack to Hyperstack' to get a composite stack. In the
dialog you define that the stack has 120 frames a 4 colors etc. If the
colors don't come out in the right order, choose a color with the top
slider and change the LUT. Control the view of the composite and export
as RGB stack with the channels tool (Ctrl shift z). This works for me
when opening folders with 3 channel tiffs in Fiji.
Hth
Christian

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Christian Goosmann
Mikroskopie
Max-Planck-Institut für Infektionsbiologie
Campus Charité Mitte
Charitéplatz 1
10117 Berlin
Tel.: [phone]

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