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Re: Using Fiji in Windows 8‏

Posted by Robert Baer on Oct 01, 2014; 3:12pm
URL: http://imagej.273.s1.nabble.com/Using-Fiji-in-Windows-8-tp5009791p5009851.html

On 9/29/2014 1:26 PM, Curtis Rueden wrote:

> Hi Robert & everyone,
>
>> This begs the question, are Fiji-saved .tifs reliable input for most
>> other programs pending the fix?
> TIFF files saved using Fiji should be valid. I'm not sure why you see any
> difference with TIFFs saved in Fiji vs. other software. The bug is a
> blanket problem opening any files passed as parameters.
>
> FWIW, the issue tracking this problem is at:
> https://github.com/imagej/imagej-legacy/issues/89
Well, the channel tool gave me some (hypothetical) insight.  Using Fiji
to convert embryos.jpg --> embyos.tif produces a 3 channel composite
image when dragged onto the Fiji icon and it displays as expected.  A
.tif file produced with the GIMP, however,  seems to produce a 4 channel
composite image when dragged onto the Fiji icon.  If this 4th channel is
checked it acts like an alpha channel that is masking the 3 color
channels.   The result is an empty image frame.  The GIMP image does
display correctly if the 3 color channels (1-3) are checked and the
fourth channel is unchecked.

Not knowing the details of .tif format importer, I leave this behavioral
clue to those who are more knowledgeable.

Rob

>
> Regards,
> Curtis
>
> On Sat, Sep 27, 2014 at 4:44 PM, Robert Baer <[hidden email]> wrote:
>
>> when a tif is double clicked, Fiji opens but not the image.
>>
>>> The issue is not Windows 8, but rather a bug in recent versions of Fiji.
>>> Until we fix it, you can try the Life-Line version, which should not
>>> exhibit the problem.
>>>
>>> Regards,
>>> Curtis
>>>
>> Interestingly, I tried my windows 8 instructions by creating an
>> embryos.tif from the Fiji sample in Fiji on Windows 8 and the instructions
>> worked fine with this test.  Garbage in - garbage out :)
>>
>> After Curtis's post, I tried a .tif saved from GIMP and had same problem
>> as OP.
>>
>> It seems that Fiji can still read it's own newly saved .tifs, [and
>> encouragingly] the GIMP can ALSO read Fiji's newly saved .tif.  Fiji just
>> behaves badly way with "some" (probably standard) .tifs, like those saved
>> by the GIMP.
>>
>> This begs the question, are Fiji-saved .tifs reliable input for most other
>> programs pending the fix?
>>
>>
>> --
>> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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Robert W. Baer, Ph.D.
Professor of Physiology
Kirksville College of Osteopathic Medicine
A T Still University of Health Sciences
800 W. Jefferson St
Kirksville, MO 63501
rbaer(at)atsu.edu

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