Re: Automated cell counter for immunostained cells

Posted by G. Esteban Fernandez on Oct 06, 2014; 12:37am
URL: http://imagej.273.s1.nabble.com/Automated-cell-counter-for-immunostained-cells-tp5009904p5009906.html

Sounds like you're not working with fluorescence.  For traditional color
stains I use the Colour Deconvolution plugin to separate the channels and
then threshold to count cells with Analyze Particles (after some processing
like Watershed segmentation). Usually this is for DAB-stained tissue
counterstained with hematoxylin, so I use the "H DAB" color matrix in
Colour Deconvolution or define my own.

To automate this I record the steps into a macro.

-Esteban
On Oct 4, 2014 9:04 AM, "csadangi" <[hidden email]> wrote:

> Hi,
>
> I want to count the number of cells in my immunostained tissues. I am
> looking for an automated counter which would do the counting once i select
> my region of interest (ROI). I have already come across the cell counter
> and
> ICTN plugin but that didn't help (ICTN) me a lot.
>
> Any help would be highly appreciated.
>
> Cheers
>
>
>
> -----
> ----
> Chinmaya
> Medical University Innsbruck
> Innsbruck
> Austria
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