Timelapse Noise Quantification

Posted by Rebecca Keller on Oct 06, 2014; 6:11pm
URL: http://imagej.273.s1.nabble.com/Timelapse-Noise-Quantification-tp5009923.html

Dear Imagers,

I have done some experiments adding Gaussian noise to my time lapses to
determine the efficacy of a certain filter, and would like to give an idea
of how much noise had been there originally. Any suggestions, then, for
measuring this "baseline experimental noise?"

I am thinking perhaps I should take a z-projected (well, "t-projected,"
really) mean image of the series, subtract it from each image in the stack,
then do a z-projected standard deviation, report the average value of this
projected image? Or instead of subtracting the first mean image, maybe I
should divide the stack by it?

Any advice here would be welcome,

Jacob Keller

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