Posted by
Krs5 on
Oct 08, 2014; 5:30pm
URL: http://imagej.273.s1.nabble.com/Multi-Plot-issues-from-ROI-Manager-tp5009964p5009965.html
Dear Stamatis,
You could try to use the macro Multi colour line profile plot (see:
https://www2.le.ac.uk/colleges/medbiopsych/facilities-and-services/cbs/lite/aif/software-1/imagej-macros) or parts of its code which allows you to collect the data from all three planes in one go. It however will still repeat the x value for each channel even when in the same "Plot Values" file.
Best wishes
Kees
Dr Ir K.R. Straatman
Senior Experimental Officer
Advanced Imaging Facility
Centre for Core Biotechnology Services
University of Leicester
http://www2.le.ac.uk/colleges/medbiopsych/facilities-and-services/cbs/lite/aif
ImageJ workshops 15 and 16 December:
http://www2.le.ac.uk/colleges/medbiopsych/facilities-and-services/cbs/lite/aif/workshops/imagej-workshops-15-and-16-December-2014
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Stamatis Pagakis
Sent: 08 October 2014 16:28
To:
[hidden email]
Subject: Multi Plot issues from ROI Manager
Hello everyone
We have RGB (3 colour fluorescence) images and we would like to plot a number of profiles along the SAME lines in each one of the R, G, B planes.
We use a composite image, ROI manager and the Multi PLot command
1) We have to do the Multi Plot command 3 times, once for each colour. Which command can we use in a macro to switch the active plane of a composite image?
Stack.setActiveChannels("000");
doesn't seem to do what we want
2) The results file, the X coordinates column is repeated for each plot. This is redundant info which makes the excel file twice as big. Is there any way to suppress this, as it can be supressed in the PlotProfile (option K) command?
3) could the output for the 3 channels be output in the same "Plot Values" file?
Thank you in advance
******************************
Stamatis Pagakis Ph.D.
Head, Biological Imaging
Biomedical Research Foundation, Academy of Athens Soranou Efessiou 4, Athens 115 27 - Greece
M: +306946644955
W: +302106597481
FAX: +302106597544
http://www.bioacademy.gr/faculty-details/HM4/Stamatis--
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