Opening images with an old OSX version

Posted by ev34 on Oct 08, 2014; 8:30pm
URL: http://imagej.273.s1.nabble.com/Opening-images-with-an-old-OSX-version-tp5009969.html

Hi,

Two related questions:

1) A student is trying to open some fluorescence images he took using the latest version of imageJ (FIJI) and the screen shows up totally black. The goal is to quantify and compare fluorescence, so we need to figure out if this issue is related to the fact that he is using a really old Mac and Mac OSX 10.6.8 or some other setting that can be changed in the software? The pictures are saved in tiff format and when he took them they were very dim, but it was still possible to see them on the computer screen attached to the microscope. I can also open them on my computer just fine (latest version of imageJ, Mac OSX 10.8.5).

2) It's been a while since I used imageJ. I installed the newest FIJI version just recently - when opening fluorescence images they look really bright and grainy, not at all like they looked on the original monitor when I took the image. Why is this and how can I view them the normal way? I also notice that when I measure fluorescence (Analyze->Measure), the mean values that I get are very different from those used in earlier imageJ versions. I imagine this is related to the extreme brightness issue?

Thank you!

~EV