Re: 3D volume measurement

Posted by Thomas Boudier on Oct 09, 2014; 1:26am
URL: http://imagej.273.s1.nabble.com/3D-volume-measurement-tp5009960p5009972.html

Dear Mauricio,

If you only want the volume of your object, just look at the histogram,
the number of white pixels (or voxels in 3D) will give you the
uncalibrated volume, multiply this number by the calibrated volume of
one voxel. For more complex analysis like shape descriptors use 3D
Object Counter or 3D Manager.

Best,

Thomas


On 08/10/14 22:39, Mauricio Aguirre Morales wrote:

> Dear Community
>
> I am trying to quantify the volume of a hypha in a stack of images. The
> 3D model was made with a CLSM and I have done some treatment
> (brightness-contrast, 3D filters, etc) and finally aquire a model that
> could be transformed into 8 bit and being black and white with "white"
> fungi and black background or just not fungi. When I use the option 3D
> viewer in the description there is a volume displayed but I wonder how
> this volume is acquired and furthermore I would really appreciate if
> somebody can tell me a better, more accurate way to measure a volume or
> even a manual selection way slide after slide or something similar.
>

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   /***************************************************************/
      Thomas Boudier, Associate Professor, UPMC,
      Université Pierre et Marie Curie, Paris, France.
      BioInformatics Institute (BII)/IPAL, Singapore.
/**************************************************************/

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