http://imagej.273.s1.nabble.com/Very-basic-macro-for-processing-lsm-images-tp5009985p5009986.html
Yes, scale bars should be properly sized. In my experience Fiji ImageJ
opens LSM files with the proper scaling.
> Hello,
>
> I've been trying to write a script that will process confocal images
> (.lsm),
> and although I've had a lot of fun trying my hand at writing a macro, I
> think I'm in over my head.
>
> To keep it simple (since I do want to learn this!), I'd like to write
> something that will process an opened image (e.g. "title.lsm") in the
> following manner:
>
> - Split channels
> - Add a scale bar to each image
> - Save the images as title.tif in the same folder as the title.lsm file
> - Merge the channels
> - Add a scale bar to the merged image
> - Save the merged image as merged-title.tif
> - Close
>
> Would a script like this add an appropriate scale bar to images that were
> taken under different magnifications?
>
> Thanks for your help!
>
>
>
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