Re: Very basic macro for processing .lsm images

Posted by Yuekan Jiao-2 on Oct 11, 2014; 3:34am
URL: http://imagej.273.s1.nabble.com/Very-basic-macro-for-processing-lsm-images-tp5009985p5010002.html

Hi,

Years ago I wrote plugins to do (batch) LSM file channel splitting
and merging (but did not code the scale bar if I remember).

Just in case you are interested.

Yuekan

On Thu, Oct 9, 2014 at 5:24 PM, G. Esteban Fernandez <
[hidden email]> wrote:

> Yes, scale bars should be properly sized. In my experience Fiji ImageJ
> opens LSM files with the proper scaling.
>
> -Esteban
> On Oct 9, 2014 3:13 PM, "atye" <[hidden email]> wrote:
>
> > Hello,
> >
> > I've been trying to write a script that will process confocal images
> > (.lsm),
> > and although I've had a lot of fun trying my hand at writing a macro, I
> > think I'm in over my head.
> >
> > To keep it simple (since I do want to learn this!), I'd like to write
> > something that will process an opened image (e.g. "title.lsm") in the
> > following manner:
> >
> > - Split channels
> > - Add a scale bar to each image
> > - Save the images as title.tif in the same folder as the title.lsm file
> > - Merge the channels
> > - Add a scale bar to the merged image
> > - Save the merged image as merged-title.tif
> > - Close
> >
> > Would a script like this add an appropriate scale bar to images that were
> > taken under different magnifications?
> >
> > Thanks for your help!
> >
> >
> >
> > --
> > View this message in context:
> >
> http://imagej.1557.x6.nabble.com/Very-basic-macro-for-processing-lsm-images-tp5009985.html
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> >
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> >
>
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