Re: DAB quantification

Posted by sarahmmph on
URL: http://imagej.273.s1.nabble.com/DAB-quantification-tp5006159p5010054.html

Hi everyone,

i have the same problem. i have samples stained with H\DAB and need to compare the results of treated and control samples. so i have 2 questions:

1- can i use threshold to determine brown area stained with DAB, then compare them?

2- which stain can be used (to avoid the DAB issue with quantification) to quantify a marker in treated and untreated samples?

Sarah M. Mosaad, M.Sc