Posted by
Sarah Mohamed on
Oct 18, 2014; 6:18pm
URL: http://imagej.273.s1.nabble.com/threshold-tp5009636p5010103.html
Dear Jacqui,
thanks for your reply, i appreciate it.
In your opinion, which stain can be used (to avoid the DAB issue with quantification) to quantify a marker in treated and untreated samples?
Kind Regards,
Sarah M. Mosaad, M.Sc.
Department of Pharmacology & Toxicology,
School of Pharmacy,
Suez Canal University, Egypt.
On Sunday, October 12, 2014 10:06 PM, Jacqui Ross <
[hidden email]> wrote:
Hi Sarah,
Just noticed that no one seems to have replied to your question. This is probably because we aren't sure what "results" you are referring to and what you mean by "threshold".
If you are measuring areas, then it's OK to use some processing to make the segmentation easier as long as you don't start to bring up some areas which were not really stained.
If you were measuring intensity for fluorescence images, then you shouldn't use any processing which changes the pixel values.
However, we know that measuring intensity/density of DAB staining is not acceptable. This has been discussed many times on the listserver.
Please read the previous thread here:
http://imagej.1557.x6.nabble.com/DAB-quantification-td5006159.html<
http://imagej.1557.x6.nabble.com/DAB-quantification-td5006159.html> in particular the comments by Gabriel Landini in reference to DAB quantification.
Kind regards,
Jacqui
Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland 1142, NEW ZEALAND
Tel: 64 9 923 7438
Fax: 64 9 373 7484
http://www.fmhs.auckland.ac.nz/sms/biru/-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Sarah Mohamed
Sent: Tuesday, 16 September 2014 10:15 p.m.
To:
[hidden email]<mailto:
[hidden email]>
Subject: threshold
Hi,
Can I manipulate with the image brightness before analysis using threshold?
I am trying to determine brown area stained with DAB using image J, i tried to use threshold and color deconvolution. my images a dark can i use photoshop to adjust background or adjust brightness (using levels or curves) ? or that will change the results?
Regards,Sarah M. Mosaad, M.Sc.
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