Re: convert 12 bit to 8 bit images faithfully

Posted by Hao Wu on Oct 21, 2014; 7:05pm
URL: http://imagej.273.s1.nabble.com/convert-12-bit-to-8-bit-images-faithfully-tp5010135p5010144.html

Really nice! Thank you so much!

Best,
Hao

On Tue, Oct 21, 2014 at 1:47 PM, Cammer, Michael <[hidden email]
> wrote:

> I think this is what you want to do:
>
> macro "Convert 12 to 8 bits linear" {
>   setMinAndMax(0, 4095);
>   call("ij.ImagePlus.setDefault16bitRange", 12);
>   run("8-bit");
> }
>
> ===========================================================================
> Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical
> Center
> Cell:  914-309-3270   note that we do not receive messages left at
> 212-263-3208
> http://ocs.med.nyu.edu/microscopy & http://microscopynotes.com/
>
>
> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Hao
> Wu
> Sent: Tuesday, October 21, 2014 1:09 PM
> To: [hidden email]
> Subject: convert 12 bit to 8 bit images faithfully
>
> Hi, everyone!
>
> I have been using ImageJ to export my 12 bit images for a long time. Today
> I find out it is not a good idea because the "8  bit" function is not
> working the way I thought. Instead of converting 0~4095 to 0~255, actually
> it converts from min~max of that particular image to 0~255. I am wondering,
> does anybody happen to know any plugins, macros or software to faithfully
> do the conversion? Thank you very much!
>
> Best,
> Hao
>
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