Automatic Nuclei Counter plug-in for ImageJ - ITCN Plugin

Posted by Jacqueline Ross on Oct 29, 2014; 4:01am
URL: http://imagej.273.s1.nabble.com/Automatic-Nuclei-Counter-plug-in-for-ImageJ-ITCN-Plugin-tp5010217.html

Hi all,

I'm looking for some help with the ITCN plugin. One of our users sent me a link to the plugin and it looks like it might be quite useful. However, I can't seem to get it to work properly.

I entered values for the Width of the nuclei and Minimum distance and have tried various threshold values without success. When I move the threshold slider nothing changes on my image. I thought it should give some indication of the nuclei that are identified.

The instructions on the website look good http://www.bioimage.ucsb.edu/automatic-nuclei-counter-plug-in-for-imagej but I can't get it to work for me even with giant nuclei that are very obvious. I haven't been able to download the example images for some reason.

I'm running Windows 7 64 bit ImageJ version 1.49f. Haven't tried it with FIJI yet.

Can anyone help me out with this?

Cheers,

Jacqui

Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland 1142, NEW ZEALAND

Tel: [phone]
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/


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